MGUS'ta azalmış GADD45A gen ifadesi seviyesi

Purpose: Monoclonal gammopathy of undetermined significance (MGUS) is a plasma cell dyscrasia. It known that MGUS has an increased risk progression to multiple myeloma (MM), and also prepares the ground for diseases such as Waldenstrom macroglobulinemia (WM), non-Hodgkin lymphoma, chronic lymphocytic leukemia (CLL). Our study aimed evaluate whether some important p53 pathway genes differ in terms expression healthy individuals.
 Materials methods: For study, bone marrow was collected from 8 individuals diagnosed with MGUS, RNA samples were isolated. Expression levels various involved compared using RT2-profiler PCR array. β-actin housekeeping gene level used normalization. The Pearson Correlation analysis Receiver Operating Characteristic (ROC) conducted.
 Results: Among whose examined this it determined only GADD45A decreased significantly control group (p=0.027). correlation data showed highly correlated 12 other (APAF1, CDK4, PCNA, BAX, CDKN2A, CASP9, CHECK2, MDM2, RB1, P53, BCL2, CHEK1) (r>0.7). In addition, according ROC analysis, detected have strong discrimination power between (AUC=0.797 p=0.015).
 Conclusion: may be useful new biomarker detect MM.